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biotinylated goat anti rabbit secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti rabbit secondary antibody
    Biotinylated Goat Anti Rabbit Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 9170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/BA-5000-1%2E5/Biotinylated+Rabbit+Anti-Goat+IgG+Antibody/pm42831432-88-4-9
    Average 96 stars, based on 9170 article reviews
    biotinylated goat anti rabbit secondary antibody - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Maladaptive Inflammatory Signaling in Old Mice Impairs Colonic Regeneration by Promoting a Sustained Fetal‐Like Epithelial State
    Article Snippet: Primary antibodies were used at the following dilutions: rat‐anti‐E‐cadherin (1:200; 13‐1900, Thermo Fisher Scientific), rabbit‐anti‐Laminin (1:250; L9393, Sigma‐Aldrich), mouse‐anti‐Ezrin (1:250; MA5‐13862, Sigma‐Aldrich), rabbit‐anti‐Ki67 (1:200; RM9106S0, Thermo Fischer Scientific), rabbit‐anti‐IFNγR1 (1:300; 10808‐1‐AP, Proteintech), rabbit‐anti‐IFNγR2 (1:300; 10266‐1‐AP, Proteintech), rabbit‐anti‐cleaved Caspase 3 (1:300; 9664, Cell Signaling Technology).

    Incubation:

    Article Title: Morphological and immunohistochemical analysis of unilateral testicular hypoplasia in an XY calf: correlation with the timing of bovine hypospadias.
    Article Snippet: For immunohistochemistry, the following primary antibodies were used: anti-androgen receptor (AR, 1:100, rabbit, sc-816; Santa Cruz Biotechnology, Dallas, TX, USA), anti-α-smooth muscle actin (αSMA, 1:500, mouse, A2547; Sigma-Aldrich, St. Louis, MO, USA), anti-FOXL2 (1:200, goat, ab5096; Abcam, Cambridge, UK), anti-GATA4 (1:100, mouse, sc-25310; Santa Cruz Biotechnology), anti-SOX9 (1:1,000, rabbit, ab5535; Merck Millipore, Burlington, MA, USA), and anti-VASA (1:1,000, rabbit, ab13840; Abcam). .. Biotin-labeled secondary antibodies (1:500, BA-5000, BA9200, and BA-1000; Vector Laboratories, Newark, CA, USA) were applied, followed by incubation with an avidin–biotin complex (PK-6100, Vector Laboratories). ..

    Article Title: Unexpected antidepressant-like effects of temozolomide in a mixed sex-cohort of adult rats: role of hippocampal FADD protein
    Article Snippet: Briefly, tissue was postfixed in 4% paraformaldehyde (Merck, Darmstadt, Germany, cat #76240) and exposed to several steps, such as peroxidase inhibition with 0.1% H 2 O 2 solution (Thermo Fisher Scientific, cat #426000010) and donkey serum as a blocking agent (Merck, cat #A7906). .. Then, sections were incubated overnight with goat anti-NeuroD (1:25000; Santa Cruz Biotechnology, CA, USA, cat #sc-1084), followed by a series of sequential incubations; secondary antibody (biotinylated anti-goat, 1:1000, Vector Laboratories, CA, USA, #BA-5000), Avidin/Biotin complex (Vectastain Elite ABC kit; Vector Laboratories, cat #PK-6100), chromogen 3,3’-diaminobenzidine (DAB; Merck, cat #D8001) with nickel chloride (Merck, cat #339350) for signal detection. .. Sections were then dehydrated in graded alcohols, immersed in xylene (Sharlab, Barcelona, Spain, cat #XI0052) and cover-slipped with Permount® (Thermo Fisher Scientific, cat #SP15-500).

    Article Title: Progesterone regulates lipid synthesis and secretion in bovine uterine epithelial cells.
    Article Snippet: .. academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag213/8833901 by guest on 30 Septem ber 2026 UN CO RR EC TE D MA NU SC RI PT and the secondary antibody (BA-5000, Vector Laboratories) was added to tissue samples and incubated for 30 min at room temperature ..

    Article Title: Reactive oxygen species-induced lipid peroxidation mediates Wnt-dependent lipodystrophy and associated skin fibrosis.
    Article Snippet: The following primary antibodies were used for immunohistochemistry: Rabbit anti-Myc tag (Abcam, ab9106; 1 : 500), Rabbit anti-4hydroxynonenal (4-HNE) antibody (Bioss bs-6313r; 1 : 300), Rabbit anti-alpha-Smooth muscle actin (a-SMA) (Abcam 124 964; 1 : 1000). .. Sections were incubated with biotinylated goat anti-rabbit secondary antibody (Vector Laboratories; 1 : 250) for 30 min at room temperature, followed by two 5-min washes in TBS. .. NeutrAvidin-HRP (Thermo Scientific, 31 030; 4 lg/mL) diluted in blocking buffer was applied for 30 min, and signal was developed using DAB substrate according to manufacturer directions (Vector Laboratories, SK-4100) (development times: 6.5 min for Myc-tag and 10 min for 4-HNE).

    Article Title: Synchronized osteochondral regeneration using a dynamic sacrificial‑phase injectable macroporous PEO‑gelatin‑GelMA hydrogel delivering BMP‑14‑engineered BMSCs
    Article Snippet: Sections were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) for 1 hr at room temperature, followed by overnight incubation at 4◦C with primary antibodies: rabbit anti-rat type II collagen (Col II, 1:200; Abcam) for cartilage matrix, and rabbit anti-rat osteocalcin (OCN, 1:200; Abcam) for bone formation. .. After PBS washes, sections were incubated with a biotinylated goat anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 hr. .. Colorimetric development was achieved using a 3,3′-diaminobenzidine (DAB) substrate kit (Vector Laboratories).

    Article Title: Renoprotective effects of perampanel against cisplatin-induced acute kidney injury: managing NLRP3-pyroptosis and enhancement of antioxidant defense.
    Article Snippet: Cisplatin is a highly effective chemotherapeutic agent used to treat various solid tumors; however, its clinical utility is limited by dose-dependent nephrotoxicity.. Perampanel, an AMPA-receptor antagonist FDA-approved anti-seizure drug, has recently shown inhibitory effects on oxidative stress and inflammasome-mediated pyroptosis in neurological damage models.. The current work examined the possible renoprotective benefits and clarified the underlying molecular signaling modified by perampanel in a cisplatin-renal injury model.

    Avidin-Biotin Assay:

    Article Title: Morphological and immunohistochemical analysis of unilateral testicular hypoplasia in an XY calf: correlation with the timing of bovine hypospadias.
    Article Snippet: For immunohistochemistry, the following primary antibodies were used: anti-androgen receptor (AR, 1:100, rabbit, sc-816; Santa Cruz Biotechnology, Dallas, TX, USA), anti-α-smooth muscle actin (αSMA, 1:500, mouse, A2547; Sigma-Aldrich, St. Louis, MO, USA), anti-FOXL2 (1:200, goat, ab5096; Abcam, Cambridge, UK), anti-GATA4 (1:100, mouse, sc-25310; Santa Cruz Biotechnology), anti-SOX9 (1:1,000, rabbit, ab5535; Merck Millipore, Burlington, MA, USA), and anti-VASA (1:1,000, rabbit, ab13840; Abcam). .. Biotin-labeled secondary antibodies (1:500, BA-5000, BA9200, and BA-1000; Vector Laboratories, Newark, CA, USA) were applied, followed by incubation with an avidin–biotin complex (PK-6100, Vector Laboratories). ..

    Article Title: Unexpected antidepressant-like effects of temozolomide in a mixed sex-cohort of adult rats: role of hippocampal FADD protein
    Article Snippet: Briefly, tissue was postfixed in 4% paraformaldehyde (Merck, Darmstadt, Germany, cat #76240) and exposed to several steps, such as peroxidase inhibition with 0.1% H 2 O 2 solution (Thermo Fisher Scientific, cat #426000010) and donkey serum as a blocking agent (Merck, cat #A7906). .. Then, sections were incubated overnight with goat anti-NeuroD (1:25000; Santa Cruz Biotechnology, CA, USA, cat #sc-1084), followed by a series of sequential incubations; secondary antibody (biotinylated anti-goat, 1:1000, Vector Laboratories, CA, USA, #BA-5000), Avidin/Biotin complex (Vectastain Elite ABC kit; Vector Laboratories, cat #PK-6100), chromogen 3,3’-diaminobenzidine (DAB; Merck, cat #D8001) with nickel chloride (Merck, cat #339350) for signal detection. .. Sections were then dehydrated in graded alcohols, immersed in xylene (Sharlab, Barcelona, Spain, cat #XI0052) and cover-slipped with Permount® (Thermo Fisher Scientific, cat #SP15-500).

    Tissue:

    Article Title: Progesterone regulates lipid synthesis and secretion in bovine uterine epithelial cells.
    Article Snippet: .. academ ic.oup.com /biolreprod/advance-article/doi/10.1093/biolre/ioag213/8833901 by guest on 30 Septem ber 2026 UN CO RR EC TE D MA NU SC RI PT and the secondary antibody (BA-5000, Vector Laboratories) was added to tissue samples and incubated for 30 min at room temperature ..

    Transcranial Magnetic Stimulation:

    Article Title: Reactive oxygen species-induced lipid peroxidation mediates Wnt-dependent lipodystrophy and associated skin fibrosis.
    Article Snippet: The following primary antibodies were used for immunohistochemistry: Rabbit anti-Myc tag (Abcam, ab9106; 1 : 500), Rabbit anti-4hydroxynonenal (4-HNE) antibody (Bioss bs-6313r; 1 : 300), Rabbit anti-alpha-Smooth muscle actin (a-SMA) (Abcam 124 964; 1 : 1000). .. Sections were incubated with biotinylated goat anti-rabbit secondary antibody (Vector Laboratories; 1 : 250) for 30 min at room temperature, followed by two 5-min washes in TBS. .. NeutrAvidin-HRP (Thermo Scientific, 31 030; 4 lg/mL) diluted in blocking buffer was applied for 30 min, and signal was developed using DAB substrate according to manufacturer directions (Vector Laboratories, SK-4100) (development times: 6.5 min for Myc-tag and 10 min for 4-HNE).



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